Sequencing of two transgenic early-flowering poplar lines confirmed vector-free single-locus T-DNA integration.

ABSTRACT: Next-generation sequencing (NGS) approaches are attractive alternatives to the PCR-based characterisation of genetically modified plants for safety assessment and labelling since NGS is highly sensitive to the detection of T-DNA inserts as well as vector backbone sequences in transgenic plants. In this study, two independent transgenic male Populus tremula lines, T193-2 and T195-1, both carrying the FLOWERING LOCUS T gene from Arabidopsis thaliana under control of a heat-inducible promoter (pHSP::AtFT) and the non-transgenic control clone W52, were further characterised by NGS and third-generation sequencing. The results support previous findings that the T-DNA was hemizygously inserted in one genomic locus of each line. However, the T-DNA insertions consist of conglomerations of one or tw

SUBMITTER: Kersten B 

PROVIDER: S-EPMC7283205 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature