A novel culture medium with reduced nutrient concentrations supports the development and viability of mouse embryos.

ABSTRACT: Further refinement of culture media is needed to improve the quality of embryos generated in vitro. Previous results from our laboratory demonstrated that uptake of nutrients by the embryo is significantly less than what is supplied in traditional culture media. Our objective was to determine the impact of reduced nutrient concentrations in culture medium on mouse embryo development, metabolism, and quality as a possible platform for next generation medium formulation. Concentrations of carbohydrates, amino acids, and vitamins could be reduced by 50% with no detrimental effects, but blastocyst development was impaired at 25% of standard nutrient provision (reduced nutrient medium; RN). Addition of pyruvate and L-lactate (+PL) to RN at 50% of standard concentrations restored blastocyst deve

SUBMITTER: Ermisch AF 

PROVIDER: S-EPMC7283311 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature