Development and Optimization of a Germination Assay and Long-Term Storage for Cannabis sativa Pollen.

ABSTRACT: Pollen viability and storage is of great interest to cannabis breeders and researchers to maintain desirable germplasm for future use in breeding or for biotechnological and gene editing applications. Here, we report a simple and efficient cryopreservation method for long-term storage of Cannabis sativa pollen. Additionally, the bicellular nature of cannabis pollen was identified using DAPI (4',6-diamidino-2-phenylindole) staining. A pollen germination assay was developed to assess cannabis pollen viability and used to demonstrate that pollen collected from different principal growth stages exhibited differential longevity. Finally, a simple and efficient method that employs pollen combined with baked whole wheat flour and subsequent desiccation under vacuum was developed for the long-term

SUBMITTER: Gaudet D 

PROVIDER: S-EPMC7284845 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature