Efficient Genome Editing in Bacillus licheniformis Mediated by a Conditional CRISPR/Cas9 System.

ABSTRACT: Bacillus licheniformis is widely used to produce multiple enzymes and chemicals in industrial fermentation. It is also an organism that is hard to genetically manipulate, which is mainly attributed to its extremely low transformation efficiency. The lack of genetic modification technology severely limits its further application. In this study, an all-in-one conditional clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 plasmid was developed for B. licheniformis with the cas9 gene under the control of a xylose-inducible promoter. By means of this design, the expression of the cas9 gene could be repressed without xylose, which significantly improved the transformation ratio from less than 0.1 cfu/μg to 2.42 cfu/μg DNA. Compared with this conditional system, a constitutive

SUBMITTER: Li Y 

PROVIDER: S-EPMC7285353 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature