ACYP2 contributes to malignant progression of glioma through promoting Ca²⁺ efflux and subsequently activating c-Myc and STAT3 signals.

ABSTRACT:

Background

Acylphosphatase 2 (ACYP2) is involved in cell differentiation, energy metabolism and hydrolysis of intracellular ion pump. It has been reported as a negative regulator in leukemia and a positive regulator in colon cancer, respectively. However, its biological role in glioma remains totally unclear.

Methods

We performed quantitative RT-PCR (qRT-PCR), immunohistochemistry (IHC) and western blot assays to evaluate ACYP2 expression. The functions of ACYP2 in glioma cells were determined by a series of in vitro and in vivo experiments, including cell proliferation, colony formation, cell cycle, apoptosis, migration, invasion and nude mouse tumorigenicity assays. In addition, western blot and co-immunoprecipitation (Co-IP) assays were used to identify its downstream tar

SUBMITTER: Li M 

PROVIDER: S-EPMC7285537 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature