Comparison of senescence-related changes between three- and two-dimensional cultured adipose-derived mesenchymal stem cells.

ABSTRACT:

Background

Adipose-derived mesenchymal stem cells (ADMSCs) have attracted widespread interest as cell-based tissue repair systems. To obtain adequate quantities of ADMSCs for therapeutic applications, extensive in vitro expansion is required. However, under current two-dimensional (2D) approaches, ADMSCs rapidly undergo replicative senescence, and cell growth is impeded and stem cell properties are eliminated by mechanisms that are poorly understood. These issues limit the extensive applications of ADMSCs. In this study, we investigated senescence-related changes in mesenchymal stem cells (MSCs) isolated from human adipose tissue in 2D and three-dimensional (3D) cultures.

Methods

We studied cell growth over a given period (21 days) to determine if modes of culture were assoc

SUBMITTER: Yin Q 

PROVIDER: S-EPMC7285747 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature