Use of Dual RNA-seq for Systems Biology Analysis of Zea mays and Aspergillus flavus Interaction.

ABSTRACT: The interaction between Aspergillus flavus and Zea mays is complex, and the identification of plant genes and pathways conferring resistance to the fungus has been challenging. Therefore, the authors undertook a systems biology approach involving dual RNA-seq to determine the simultaneous response from the host and the pathogen. What was dramatically highlighted in the analysis is the uniformity in the development patterns of gene expression of the host and the pathogen during infection. This led to the development of a "stage of infection index" that was subsequently used to categorize the samples before down-stream system biology analysis. Additionally, we were able to ascertain that key maize genes in pathways such as the jasmonate, ethylene and ROS pathways, were up-regul

SUBMITTER: Musungu B 

PROVIDER: S-EPMC7285840 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature