Trans-NanoSim characterizes and simulates nanopore RNA-sequencing data.

ABSTRACT:

Background

Compared with second-generation sequencing technologies, third-generation single-molecule RNA sequencing has unprecedented advantages; the long reads it generates facilitate isoform-level transcript characterization. In particular, the Oxford Nanopore Technology sequencing platforms have become more popular in recent years owing to their relatively high affordability and portability compared with other third-generation sequencing technologies. To aid the development of analytical tools that leverage the power of this technology, simulated data provide a cost-effective solution with ground truth. However, a nanopore sequence simulator targeting transcriptomic data is not available yet.

Findings

We introduce Trans-NanoSim, a tool that simulates reads with technical

SUBMITTER: Hafezqorani S 

PROVIDER: S-EPMC7285873 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature