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Development of a TaqMan qPCR assay for detection of Alexandrium spp and application to harmful algal bloom monitoring.


ABSTRACT: The Genus Alexandrium is a widespread dinoflagellate marine phytoplankton that is the primary causative organism causing Paralytic Shellfish Poisoning (PSP) intoxications in European waters. EU food safety directives specify that EU Member States must implement a routine monitoring programme to mitigate risks associated with bio-accumulation of biotoxins by bivalve shellfish, such as those produced by Alexandrium. This strategic drive comprises of both direct testing of bivalve flesh for the presence of regulated toxins and an early warning phytoplankton monitoring programme. In the UK the flesh testing moved away from animal bio-assays to analytical chemistry techniques, whereas phytoplankton monitoring methods have seen little technological advancement since implementation.

SUBMITTER: Hatfield RG 

PROVIDER: S-EPMC7285902 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature