Isolation and characterization of cytotoxic and insulin-releasing components from the venom of the black-necked spitting cobra Naja nigricollis (Elapidae).

ABSTRACT: Four peptides with cytotoxic activity against BRIN-BD11 rat clonal β-cells were purified from the venom of the black-necked spitting cobra Naja nigricollis using reversed-phase HPLC. The peptides were identified as members of the three-finger superfamily of snake toxins by ESI-MS/MS sequencing of tryptic peptides. The most potent peptide (cytotoxin-1N) showed strong cytotoxic activity against three human tumor-derived cell lines (LC₅₀ = 0.8 ± 0.2 μM for A549 non-small cell lung adenocarcinoma cells; LC₅₀ = 7 ± 1 μM for MDA-MB-231 breast adenocarcinoma cells; and LC₅₀ = 9 ± 1 μM for HT-29 colorectal adenocarcinoma cells). However, all the peptides were to varying degrees cytotoxic against HUVEC human umbilical vein endothelial cells (LC₅₀ i

SUBMITTER: Conlon JM 

PROVIDER: S-EPMC7285909 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature