ALCAM (CD166) as a gene expression marker for human mesenchymal stromal cell characterisation.

ABSTRACT:

Background

Human mesenchymal stromal cells (MSCs) phenotypically share their positive expression of the International Society for Cell and Gene Therapy (ISCT) markers CD73, CD90 and CD105 with fibroblasts. Fibroblasts are often co-isolated as an unwanted by-product from biopsy and they can rapidly overgrow the MSCs in culture. Indeed, many other surface markers have been proposed, though no unique MSC specific marker has been identified yet. Quantitative PCR (qPCR) is a precise, efficient and rapid method for gene expression analysis. To identify a marker suitable for accurate MSC characterisation, qPCR was exploited.

Methods and results

Two commercially obtained bone marrow (BM) derived MSCs and an hTERT immortalised BM-MSC line (MSC-TERT) have been cultured for different d

SUBMITTER: Brinkhof B 

PROVIDER: S-EPMC7285916 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature