Electrophysiological properties of basal forebrain cholinergic neurons identified by genetic and optogenetic tagging.

ABSTRACT: Recent developments in the generation of neuronal population-specific, genetically modified mouse lines have allowed precise identification and selective stimulation of cholinergic neurons in vivo. Although considerably less laborious than studies conducted with post hoc identification of cholinergic neurons by immunostaining, it is not known whether the genetically based labeling procedures that permit in vivo identification are electrophysiologically benign. In this study, we use mice carrying a bacterial artificial chromosome transgene that drives expression of a tau-green fluorescent fusion protein specifically in cholinergic neurons. This allowed us to visualize basal forebrain cholinergic neurons in acute slice preparations. Using whole cell, patch clamp electrophysiological recordin

SUBMITTER: Lopez-Hernandez GY 

PROVIDER: S-EPMC7286072 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature