One-step multiplex TaqMan probe-based method for real-time PCR detection of four canine diarrhea viruses.

ABSTRACT: Viral canine diarrhea has high morbidity and mortality and is prevalent worldwide, resulting in severe economic and spiritual losses to pet owners. However, diarrhea pathogens have similar clinical symptoms and are difficult to diagnose clinically. Thus, fast and accurate diagnostic methods are of great significance for prevention and accurate treatment. In this study, we developed a one-step multiplex TaqMan probe-based real-time PCR for the differential diagnosis of four viruses causing canine diarrhea including, CPV (Canine Parvovirus), CCoV (Canine Coronavirus), CAstV (Canine Astrovirus), and CaKoV (Canine Kobuviruses). The limit of detection was up to 10² copies/μL and performed well with high sensitivity and specificity. This assay was optimized and used to identify possib

SUBMITTER: Wang R 

PROVIDER: S-EPMC7286240 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature