Inhibition of the prostaglandin D₂-GPR44/DP2 axis improves human islet survival and function.

ABSTRACT:

Aims/hypothesis

Inflammatory signals and increased prostaglandin synthesis play a role during the development of diabetes. The prostaglandin D₂ (PGD₂) receptor, GPR44/DP2, is highly expressed in human islets and activation of the pathway results in impaired insulin secretion. The role of GPR44 activation on islet function and survival rate during chronic hyperglycaemic conditions is not known. In this study, we investigate GPR44 inhibition by using a selective GPR44 antagonist (AZ8154) in human islets both in vitro and in vivo in diabetic mice transplanted with human islets.

Methods

Human islets were exposed to PGD₂ or proinflammatory cytokines in vitro to investigate the effect of GPR44 inhibition on islet survival rate. In addition, the mol

SUBMITTER: Abadpour S 

PROVIDER: S-EPMC7286861 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature