Reference Genes for Expression Studies in Human CD8⁺ Naive and Effector Memory T Cells under Resting and Activating Conditions.

ABSTRACT: Reverse-transcription quantitative real-time polymerase chain reaction (RT-qPCR) is widely used for mRNA quantification. To accurately measure changing gene transcript levels under different experimental conditions, the use of appropriate reference gene transcripts is instrumental. In T cell immunology, suitable reference genes have been reported for bulk CD4⁺ and CD8⁺ T cells. However, many CD4⁺ and CD8⁺ T cell subsets have been described in the past. Although they respond differently to given activation stimuli, proper validation of suitable reference genes in these subsets is lacking. In this study, we evaluated twelve commonly used reference gene products in human naïve (NV) and effector memory (EM) CD8⁺ T cells under non-activated

SUBMITTER: Geigges M 

PROVIDER: S-EPMC7286888 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature