Glucose transporter 1 is important for the glycolytic metabolism of human endometrial stromal cells in hypoxic environment.

ABSTRACT:

Aim

The study aimed to elucidate the glycolytic metabolism of human endometrial stromal cells (hESCs) in hypoxic environment.

Main methods

The hESCs were cultured in hypoxic environment, and their metabolic pathways were analyzed using metabolomics. We assessed glucose uptake using 2-deoxyglucose (2-DG) assay. The expression of glucose transporters (GLUTs) required for glucose uptake was determined using real-time quantitative polymerase chain reaction (qPCR) and western blotting. Furthermore, we knocked down GLUT1 and examined the uptake of 2-DG.

Key findings

Under hypoxia, glucose-6-phosphate, fructose-6-phosphate, and fructose-1,6-diphosphate were significantly elevated in hESCs (P < 0.05). This finding indicated enhancement in glycolysis. The volume of glucose upt

SUBMITTER: Kido T 

PROVIDER: S-EPMC7286975 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature