Role of mitochondrial fission-related genes in mitochondrial morphology and energy metabolism in mouse embryonic stem cells.

ABSTRACT: Mitochondria, the major organelles that produce energy for cell survival and function, dynamically change their morphology via fusion and fission, a process called mitochondrial dynamics. The details of the underlying mechanism of mitochondrial dynamics have not yet been elucidated. Here, we aimed to investigate the function of mitochondrial fission genes in embryonic stem cells (ESCs). To this end, we generated homozygous knockout ESC lines, namely, Fis1^-/-, Mff^-/-, and Dnm1l^-/- ESCs, using the CRISPR-Cas9 system. Interestingly, the Fis1^-/-, Mff^-/-, and Dnm1l^-/- ESCs showed normal morphology, self-renewal, and the ability to differentiate into all three germ layers in vitro. However, transmission electron microscopy showed

SUBMITTER: Seo BJ 

PROVIDER: S-EPMC7286981 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature