Fungal-like particles and macrophage-conditioned medium are inflammatory elicitors for 3T3-L1 adipocytes.

ABSTRACT: Adipocytes from white-adipose tissue are known to produce inflammatory cytokines, which play a major role in energy balance and metabolism. While they can respond to pathogen-associated molecular pattern (PAMPs) such as lipopolysaccharide (LPS) from bacteria, it is not known whether adipocytes can be stimulated by fungal cells. Previously, adipocytes were shown to produce toll-like receptor 2 (TLR2), a β-glucan receptor, suggesting that they could respond to β-glucan on the fungal cell wall. In this study, we show that heat-killed yeast induce an inflammatory response in adipocytes. Using fungal-like particles, namely laminarin-coated beads (LCB), we find that these particles trigger the expression of many key inflammatory genes in dose- and time-dependent fashions in adipocytes. These res

SUBMITTER: Jakkawanpitak C 

PROVIDER: S-EPMC7287055 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature