Accounting for variability in ion current recordings using a mathematical model of artefacts in voltage-clamp experiments.

ABSTRACT: Mathematical models of ion channels, which constitute indispensable components of action potential models, are commonly constructed by fitting to whole-cell patch-clamp data. In a previous study, we fitted cell-specific models to hERG1a (Kv11.1) recordings simultaneously measured using an automated high-throughput system, and studied cell-cell variability by inspecting the resulting model parameters. However, the origin of the observed variability was not identified. Here, we study the source of variability by constructing a model that describes not just ion current dynamics, but the entire voltage-clamp experiment. The experimental artefact components of the model include: series resistance, membrane and pipette capacitance, voltage offsets, imperfect compensations made by the amplifier f

SUBMITTER: Lei CL 

PROVIDER: S-EPMC7287334 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature