'What I cannot create, I do not understand': functionally validated synergism of metabolic and target site insecticide resistance.

ABSTRACT: The putative synergistic action of target-site mutations and enhanced detoxification in pyrethroid resistance in insects has been hypothesized as a major evolutionary mechanism responsible for dramatic consequences in malaria incidence and crop production. Combining genetic transformation and CRISPR/Cas9 genome modification, we generated transgenic Drosophila lines expressing pyrethroid metabolizing P450 enzymes in a genetic background along with engineered mutations in the voltage-gated sodium channel (para) known to confer target-site resistance. Genotypes expressing the yellow fever mosquito Aedes aegypti Cyp9J28 while also bearing the para^V1016G mutation displayed substantially greater resistance ratio (RR) against deltamethrin than the product of

SUBMITTER: Samantsidis GR 

PROVIDER: S-EPMC7287358 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature