Efficient targeted mutagenesis in allotetraploid sweet basil by CRISPR/Cas9.

ABSTRACT: Sweet basil (Ocimum basilicum) is an economically important herb and its global production is threatened by basil downy mildew caused by the obligate biotrophic oomycete Peronospora belbahrii. Effective tools are required for functional understanding of its genes involved in synthesis of valuable secondary metabolites in essential oil and disease resistance, and breeding for varieties with improved traits. Clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 gene editing technology has revolutionized crop breeding and functional genomics. The applicability and efficacy of this genomic tool in the allotetraploid sweet basil were tested by editing a potential susceptibility (S) gene ObDMR1, the basil homolog of Arabidopsis DMR1 (Downy M

SUBMITTER: Navet N 

PROVIDER: S-EPMC7287412 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature