⁸⁹Zr-Labeled AR20.5: A MUC1-Targeting ImmunoPET Probe.

ABSTRACT: High expression levels of the tumor-associated antigen MUC1 have been correlated with tumor aggressiveness, poor response to therapy, and poor survival in several tumor types, including breast, pancreatic, and epithelial ovarian cancer. Herein, we report the synthesis, characterization, and in vivo evaluation of a novel radioimmunoconjugate for the immuno-positron emission tomography (immunoPET) imaging of MUC1 expression based on the AR20.5 antibody. To this end, we modified AR20.5 with the chelator desferrioxamine (DFO) and labeled it with the positron-emitting radiometal zirconium-89 (t_1/2 ~3.3 d) to produce [⁸⁹Zr]Zr-DFO-AR20.5. In subsequent in vivo experiments in athymic nude mice bearing subcutaneous MUC1-expressing ovarian cancer xenografts, [⁸⁹Zr]Zr

SUBMITTER: Fung K 

PROVIDER: S-EPMC7287814 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature