Near-Infrared Dual-Emission Ratiometric Fluorescence Imaging Nanoprobe for Real-Time Tracing the Generation of Endogenous Peroxynitrite in Single Living Cells and In Vivo.

ABSTRACT: Peroxynitrite (ONOO^-) is a highly reactive nitrogen species with potent oxidant and nitrating properties. Its excessive generation can cause DNA and protein damage, thereby contributing to cell injury, and it is closely related to the development of many diseases. Thus, there is an urgent need for a reliable method to determine changes in the steady-state levels of ONOO^- in vivo. Ratiometric imaging, due to its built-in self-calibration system, can reduce artifacts and enable reliable in vivo imaging. In this study, we designed and prepared near-infrared (NIR) biomass quantum dots (NI-BQDs) and covalently coupled them with the NIR dye Cyanine7 (Cy7) to construct an NIR dual-emission nanoprobe (NI-BQD-Cy7) for real-time tracing the generation of endogenous ONOO^-</

SUBMITTER: Lin P 

PROVIDER: S-EPMC7288700 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature