Glucose Microsensor with Covalently Immobilized Glucose Oxidase for Probing Bacterial Glucose Uptake by Scanning Electrochemical Microscopy.

ABSTRACT: We have developed a new dual-tip glucose sensing scanning electrochemical microcopy (SECM) probe by covalently immobilizing the glucose oxidase (GOD) enzyme onto an ultramicro electrode (UME) to measure the local glucose consumption of Streptococcus mutans (S. mutans) biofilms. GOD was immobilized on a novel enzyme immobilization matrix of functionalized multiwalled carbon nanotubes (f-MWCNTs) and 1-butyl-4-methylpyridinium hexafluorophosphate (ionic liquid/IL) packed into the etched Pt UME. The highly selective GOD-based SECM tip showed a high current density of 94.44 (±18.55) μA·mM^-1·cm^-2 from 0.10 to 1.0 mM at 37 °C as a result of the synergetic effects of f-MWCNTs and ionic liquid. The detection limit of the new 25 μm diameter glucose sensor is 10.0

SUBMITTER: Jayathilake NM 

PROVIDER: S-EPMC7288757 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature