Oxidative Stress Alters Angiogenic and Antimicrobial Content of Extracellular Vesicles and Improves Flap Survival.

ABSTRACT: Extracellular vesicles (EVs) secreted from adipose-derived mesenchymal stem cells (ADSCs) (ADSC-EVs) improve flap survival after ischemia-reperfusion injury. Exposure of parent ADSCs to oxidative stress has been shown to enhance this effect, but mechanisms are unclear. We aimed to determine whether angiogenesis-promoting protein and microRNA (miRNA) content is altered in EVs after preconditioning with hydrogen peroxide (H₂O₂ ADSC-EVs) and whether H₂O₂ ADSC-EVs can increase viability of random pattern skin flaps.

Methods

EVs secreted by human ADSCs were isolated after culture in EV-depleted medium ± H₂O₂. Nanoparticle tracking analysis determined size and concentration of purified EVs. Mass spectrometry and small RNA n

SUBMITTER: Mayo JS 

PROVIDER: S-EPMC7288884 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature