Arrangement of the Polymerase Complexes inside a Nine-Segmented dsRNA Virus.

ABSTRACT: Members of the family Reoviridae package several copies of the viral polymerase complex into their capsid to carry out replication and transcription within viral particles. Classical single-particle reconstruction encounters difficulties resolving structures such as the intraparticle polymerase complex because refinement can converge to an incorrect map and because the map could depict a nonrepresentative subset of particles or an average of heterogeneous particles. Using the nine-segmented Fako virus, we tested hypotheses for the arrangement and number of polymerase complexes within the virion by measuring how well each hypothesis describes the set of cryoelectron microscopy images of individual viral particles. We find that the polymerase complex in Fako virus binds at ten possible sites

SUBMITTER: Kaelber JT 

PROVIDER: S-EPMC7289189 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature