Systematic comparison of single-cell and single-nucleus RNA-sequencing methods.

ABSTRACT: The scale and capabilities of single-cell RNA-sequencing methods have expanded rapidly in recent years, enabling major discoveries and large-scale cell mapping efforts. However, these methods have not been systematically and comprehensively benchmarked. Here, we directly compare seven methods for single-cell and/or single-nucleus profiling-selecting representative methods based on their usage and our expertise and resources to prepare libraries-including two low-throughput and five high-throughput methods. We tested the methods on three types of samples: cell lines, peripheral blood mononuclear cells and brain tissue, generating 36 libraries in six separate experiments in a single center. To directly compare the methods and avoid processing differences introduced by the existing pipelines,

SUBMITTER: Ding J 

PROVIDER: S-EPMC7289686 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature