Detachment of cell sheets from clinically ubiquitous cell culture vessels by ultrasonic vibration.

ABSTRACT: Proteinases that digest the extracellular matrix are usually used to harvest cells from culture vessels in a general culture process, which lowers the initial adhesion rate in regenerative medicine. Cell sheet engineering is one of the most important technologies in this field, especially for transplantation, because fabricated cell sheets have rich extracellular matrixes providing strong initial adhesion. Current cell sheet fabrication relies on temperature-responsive polymer-coated dishes. Cells are cultured on such specialized dishes and subjected to low temperature. Thus, we developed a simple but versatile cell sheet fabrication method using ubiquitous culture dishes/flasks without any coating or temperature modulation. Confluent mouse myoblasts (C2C12 cell line) were exposed to ultra

SUBMITTER: Imashiro C 

PROVIDER: S-EPMC7289836 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature