Selective Induction of Human Autonomic Neurons Enables Precise Control of Cardiomyocyte Beating.

ABSTRACT: The autonomic nervous system (ANS) regulates tissue homeostasis and remodelling through antagonistic effects of noradrenergic sympathetic and cholinergic parasympathetic signalling. Despite numerous reports on the induction of sympathetic neurons from human pluripotent stem cells (hPSCs), no induction methods have effectively derived cholinergic parasympathetic neurons from hPSCs. Considering the antagonistic effects of noradrenergic and cholinergic inputs on target organs, both sympathetic and parasympathetic neurons are expected to be induced. This study aimed to develop a stepwise chemical induction method to induce sympathetic-like and parasympathetic-like ANS neurons. Autonomic specification was achieved through restricting signals inducing sensory or enteric neurogenesis and activati

SUBMITTER: Takayama Y 

PROVIDER: S-EPMC7289887 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature