Oxymatrine Inhibits Renal Cell Carcinoma Progression by Suppressing β-Catenin Expression.

ABSTRACT:

Aims

Oxymatrine (OMT) has been identified to possess immunomodulatory, antiinflammatory and anticancer properties. This study aimed to investigate its precise function and the underlying molecular mechanisms in renal cell carcinoma progression.

Methods

The antineoplastic effect of oxymatrine was investigated by CCK-8 assay, cell cycle analysis, apoptosis assay, wound healing experiment, transwell assay, and drug-sensitivity analysis in renal cancer cells following oxymatrine treatment. The modulation of oxymatrine on β-catenin was analyzed through western blot and immunofluorescence assay. β-catenin overexpression was employed to determine the key role of β-catenin in oxymatrine-inhibited renal cell carcinoma in vitro. In addition, animal model was established to inve

SUBMITTER: Jin Y 

PROVIDER: S-EPMC7289957 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature