Glucose metabolism pattern of peripheral blood immune cells in myasthenia gravis patients.

ABSTRACT:

Background

We investigated the correlation between glucose metabolism patterns of different immune cells and the metabolic regulatory signaling pathways in myasthenia gravis (MG) and aimed to identify therapeutic targets for MG.

Methods

We isolated peripheral blood mononuclear cells (PBMCs) and sorted CD19⁺B cells, dendritic cells (DCs), CD4⁺ T cells, CD8⁺ T cells, CD4⁺CD25⁺ regulatory T cells (Tregs), CD4⁺CD25^-T cells, and T helper (Th) cells such as Th1, Th2, and Th17 cells. Then, we detected the expression levels of PI3K/AKT/mTOR-HIF-1α, GLUT1, hexokinase (HK), phosphofructokinase (PFK), and pyruvate kinase (PK) by RT-PCR, measured the oxygen consumption rate and extracellular acidification rate

SUBMITTER: Li Z 

PROVIDER: S-EPMC7290526 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature