Suppression of CaMKIIβ Inhibits ANO1-Mediated Glioblastoma Progression.

ABSTRACT: ANO1, a Ca²⁺-activated chloride channel, is highly expressed in glioblastoma cells and its surface expression is involved in their migration and invasion. However, the regulation of ANO1 surface expression in glioblastoma cells is largely unknown. In this study, we found that Ca²⁺/Calmodulin-dependent protein kinase II (CaMKII) β specifically enhances the surface expression and channel activity of ANO1 in U251 glioblastoma cells. When KN-93, a CaMKII inhibitor, was used to treat U251 cells, the surface expression and channel activity of ANO1 were significantly reduced. Only CaMKIIβ, among the four CaMKII isoforms, increased the surface expression and channel activity of ANO1 in a heterologous expression system. Additionally, gene silencing of CaMKIIβ suppressed the su

SUBMITTER: Sim KM 

PROVIDER: S-EPMC7290681 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature