Comparative Application of BioID and TurboID for Protein-Proximity Biotinylation.

ABSTRACT: BioID is a well-established method for identifying protein-protein interactions and has been utilized within live cells and several animal models. However, the conventional labeling period requires 15-18 h for robust biotinylation which may not be ideal for some applications. Recently, two new ligases termed TurboID and miniTurbo were developed using directed evolution of the BioID ligase and were able to produce robust biotinylation following a 10 min incubation with excess biotin. However, there is reported concern about inducibility of biotinylation, cellular toxicity, and ligase stability. To further investigate the practical applications of TurboID and ascertain strengths and weaknesses compared to BioID, we developed several stable cell lines expressing BioID and TurboID fusion prote

SUBMITTER: May DG 

PROVIDER: S-EPMC7290721 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature