Cell Cycle-Dependent Dynamics of the Golgi-Centrosome Association in Motile Cells.

ABSTRACT: Here, we characterize spatial distribution of the Golgi complex in human cells. In contrast to the prevailing view that the Golgi compactly surrounds the centrosome throughout interphase, we observe characteristic differences in the morphology of Golgi ribbons and their association with the centrosome during various periods of the cell cycle. The compact Golgi complex is typical in G1; during S-phase, Golgi ribbons lose their association with the centrosome and extend along the nuclear envelope to largely encircle the nucleus in G2. Interestingly, pre-mitotic separation of duplicated centrosomes always occurs after dissociation from the Golgi. Shortly before the nuclear envelope breakdown, scattered Golgi ribbons reassociate with the separated centrosomes restoring two compact Golgi comple

SUBMITTER: Frye K 

PROVIDER: S-EPMC7290758 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature