Digital Spindle: A New Way to Explore Mitotic Functions by Whole Cell Data Collection and a Computational Approach.

ABSTRACT: From cells to organisms, every living system is three-dimensional (3D), but the performance of fluorescence microscopy has been largely limited when attempting to obtain an overview of systems' dynamic processes in three dimensions. Recently, advanced light-sheet illumination technologies, allowing drastic improvement in spatial discrimination, volumetric imaging times, and phototoxicity/photobleaching, have been making live imaging to collect precise and reliable 3D information increasingly feasible. In particular, lattice light-sheet microscopy (LLSM), using an ultrathin light-sheet, enables whole-cell 3D live imaging of cellular processes, including mitosis, at unprecedented spatiotemporal resolution for extended periods of time. This technology produces immense and complex data, includ

SUBMITTER: Yamashita N 

PROVIDER: S-EPMC7291015 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature