stim2b Knockout Induces Hyperactivity and Susceptibility to Seizures in Zebrafish Larvae.

ABSTRACT: In neurons, stromal interaction molecule (STIM) proteins regulate store-operated Ca²⁺ entry (SOCE) and are involved in calcium signaling pathways. However, STIM activity in neurological diseases is unclear and should be clarified by studies that are performed in vivo rather than in cultured cells in vitro. The present study investigated the role of neuronal Stim2b protein in zebrafish. We generated stim2b knockout zebrafish, which were fertile and had a regular lifespan. Using various behavioral tests, we found that stim2b^-/- zebrafish larvae were hyperactive compared with wild-type fish. The mutants exhibited increases in mobility and thigmotaxis and disruptions of phototaxis. They were also more sensitive to pentylenetetrazol and glutamate treatments.

SUBMITTER: Wasilewska I 

PROVIDER: S-EPMC7291033 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature