Green Bees: Reverse Genetic Analysis of Deformed Wing Virus Transmission, Replication, and Tropism.

ABSTRACT: Environmental and agricultural pollination services by honey bees, Apis mellifera, and honey production are compromised by high levels of annual colony losses globally. The majority are associated with disease caused by deformed wing virus (DWV), a positive-strand RNA virus, exacerbated by the ectoparasitic mite Varroa destructor. To improve honey bee health, a better understanding of virus transmission and pathogenesis is needed which requires the development of tools to study virus replication, transmission, and localisation. We report the use of reverse genetic (RG) systems for the predominant genetically distinct variants of DWV to address these questions. All RG-recovered viruses replicate within 24 h post-inoculation of pupae and could recapitulate the characteristic sy

SUBMITTER: Gusachenko ON 

PROVIDER: S-EPMC7291132 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature