Expression of Free Fatty Acid Receptor 2 by Dendritic Cells Prevents Their Expression of Interleukin 27 and Is Required for Maintenance of Mucosal Barrier and Immune Response Against Colorectal Tumors in Mice.

ABSTRACT:

Background & aims

Intestinal microbes and their metabolites affect the development of colorectal cancer (CRC). Short-chain fatty acids are metabolites generated by intestinal microbes from dietary fiber. We investigated the mechanisms by which free fatty acid receptor 2 (FFAR2), a receptor for short-chain fatty acids that can affect the composition of the intestinal microbiome, contributes to the pathogenesis of CRC.

Methods

We performed studies with Apc^Min/+ mice, Apc^Min/+Ffar2^-/- mice, mice with conditional disruption of Ffar2 in dendritic cells (DCs) (Ffar2^fl/flCD11c-Cre mice), Apc^Min/+Ffar2^fl/flCD11c-Cre mice, and Ffar2^fl/fl mice (controls); some mice were given dextran sodium sulfate to induce

SUBMITTER: Lavoie S 

PROVIDER: S-EPMC7291292 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature