Validation and quantification of major biomarkers in 'Mahasudarshan Churna'- an ayurvedic polyherbal formulation through high-performance thin-layer chromatography.

ABSTRACT:

Background

Mahasudarshan Churna (MC) is a polyherbal Ayurvedic medicine that is employed in fever (especially chronic type), cold and malaria, improvement of digestion and appetite, removes toxins from the blood, boosts immunity and protects against common bacterial infections.

Methods

Validation and quantification of oleanolic acid (OA), ursolic acid (UA), mangiferin (M), gallic acid (GA), quercetin (Q) and curcumin (C) in commercial MC formulations by HPTLC method. Mobile phase, hexane: ethyl acetate: acetone (16.4: 3.6: 0.2, v/v) was used for the separation of OA and UA; ethyl acetate: glacial acetic acid: formic acid: water (20: 2.2: 2.2: 5.2 v/v) for the development of M; and toluene: ethyl acetate: formic acid (13.5: 9: 0.6 v/v) for the separation of GA, Q and C in cru

SUBMITTER: Kaur P 

PROVIDER: S-EPMC7291524 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature