Disease Activity-Associated Alteration of mRNA m⁵ C Methylation in CD4⁺ T Cells of Systemic Lupus Erythematosus.

ABSTRACT: Epigenetic processes including RNA methylation, post-translational modifications, and non-coding RNA expression have been associated with the heritable risks of systemic lupus erythematosus (SLE). In this study, we aimed to explore the dysregulated expression of 5-methylcytosine (m⁵C) in CD4⁺ T cells from patients with SLE and the potential function of affected mRNAs in SLE pathogenesis. mRNA methylation profiles were ascertained through chromatography-coupled triple quadrupole mass spectrometry in CD4⁺ T cells from two pools of patients with SLE exhibiting stable activity, two pools with moderate-to-major activity, and two pools of healthy controls (HCs). Simultaneously, mRNA methylation profiles and expression profiling were performed using RNA-Bis-Seq an

SUBMITTER: Guo G 

PROVIDER: S-EPMC7291606 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature