Elimination of "kitome" and "splashome" contamination results in lack of detection of a unique placental microbiome.

ABSTRACT:

Background

A placental microbiome, which may be altered in gestational diabetes mellitus (GDM), has been described. However, publications raising doubts about the existence of a placental microbiome that is different than contaminants in DNA extraction kits and reagents ("kitomes") have emerged. The aims of this study were to confirm the existence of a placental microbiome distinct from contaminants and determine if it is altered in GDM mothers.

Results

We first enrolled normal weight, obese and GDM mothers (N = 17) at term elective cesarean section delivery in a pilot case control study. Bacterial DNA was extracted from placental parenchyma, maternal and cord blood, maternal vaginal-rectal swabs, and positive and negative controls with the standard Qiagen/MoBio Power Soil k

SUBMITTER: Olomu IN 

PROVIDER: S-EPMC7291729 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature