Metabolic engineering of Escherichia coli for efficient production of L-alanyl-L-glutamine.

ABSTRACT:

Background

L-Alanyl-L-glutamine (AQ) is a functional dipeptide with high water solubility, good thermal stability and high bioavailability. It is widely used in clinical treatment, post-operative rehabilitation, sports health care and other fields. AQ is mainly produced via chemical synthesis which is complicated, time-consuming, labor-intensive, and have a low yield accompanied with the generation of by-products. It is therefore highly desirable to develop an efficient biotechnological process for the industrial production of AQ.

Results

A metabolically engineered E. coli strain for AQ production was developed by over-expressing L-amino acid α-ligase (BacD) from Bacillus subtilis, and inactivating the peptidases PepA, PepB, PepD, and PepN, as well as the dipeptide transport

SUBMITTER: Zhu J 

PROVIDER: S-EPMC7291740 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature