A novel patient-derived organoids-based xenografts model for preclinical drug response testing in patients with colorectal liver metastases.

ABSTRACT:

Backgrounds

Cancer-related mortality in patients with colorectal cancer (CRC) is predominantly caused by development of colorectal liver metastases (CLMs). How to screen the sensitive chemotherapy and targeted therapy is the key element to improve the prognosis of CLMs patients. The study aims to develop patient-derived organoids-based xenografted liver metastases (PDOX-LM) model of CRC, to recapitulate the clinical drug response.

Methods

We transplanted human CRC primary tumor derived organoids in murine spleen to obtain xenografted liver metastases in murine liver. Immunohistochemistry (IHC) staining, whole-exome and RNA sequencing, and drug response testing were utilized to identify the homogeneity in biological and genetic characteristics, and drug response between the P

SUBMITTER: Jian M 

PROVIDER: S-EPMC7291745 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature