Fecal MicroRNAs Show Promise as Noninvasive Crohn's Disease Biomarkers.

ABSTRACT:

Background

Short non-coding microRNAs (miRNAs) are involved in various cellular processes during disease progression of Crohn's disease (CD) and remarkably stable in feces, which make them attractive biomarker candidates for reflecting intestinal inflammatory processes. Here we investigated the potential of fecal miRNAs as noninvasive and translational CD biomarkers.

Methods

MiRNAs were screened in feces of 52 patients with CD and 15 healthy controls using RNA sequencing and the results were confirmed by PCR. The relationship between fecal miRNA levels and the clinical CD activity index (CDAI) or CD endoscopic index of severity (CDEIS) was explored, respectively. Additionally, fecal miRNAs were investigated in dextran sodium sulfate, adoptive T-cell transfer, and Helicoba

SUBMITTER: Wohnhaas CT 

PROVIDER: S-EPMC7291945 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature