Determination of lamotrigine in human plasma using liquid chromatography-tandem mass spectrometry.

ABSTRACT:

Aim

Lamotrigine (LTG) is a widely used anti-epileptic drug that is administered to avoid seizures and to maintain seizure-free status. However, several factors reportedly cause individual differences of plasma LTG levels, and the therapeutic target range of LTG varies between individuals. Thus, to optimize effective doses of LTG, we developed a rapid and simple method for determining plasma LTG concentrations.

Methods

Lamotrigine and the internal standard papaverine were extracted from human plasma using solid-phase extraction. After filtration, 5-μL aliquots of final samples were injected into the liquid chromatography-tandem mass spectrometry instrument and LTG and internal standard were separated using a Cadenza CD-C18 column (100 × 2 mm, 3 μm) with 0.1% formic acid in wa

SUBMITTER: Itabashi S 

PROVIDER: S-EPMC7292279 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature