Evaluation of the usefulness of saliva for DNA methylation analysis in cohort studies.

ABSTRACT:

Introduction

Epigenetic information such as DNA methylation is a useful biomarker that reflects complex gene-environmental interaction. Peripheral tissues such as blood and saliva are commonly collected as the source of genomic DNA in cohort studies. Epigenetic studies mainly use blood, while a few studies have addressed the epigenetic characteristics of saliva.

Methods

The effects of methods for DNA extraction and purification from saliva on DNA methylation were surveyed using Illumina Infinium HumanMethylation450 BeadChip. Using 386 661 probes, DNA methylation differences between blood and saliva from 22 healthy volunteers, and their functional and structural characteristics were examined. CpG sites with DNA methylation levels showing large interindividual variations in bl

SUBMITTER: Murata Y 

PROVIDER: S-EPMC7292296 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature