Induced neuronal activity does not attenuate amyloid beta-induced synaptic loss in vitro.

ABSTRACT:

Aim

The accumulation of amyloid beta (Aβ) is one of the characteristics of Alzheimer's disease. The excessive accumulation of Aβ has been suggested to result in a decrease in the number of synapses. Although the number of synapses is generally modulated by neuronal activity, whether neuronal activity affects Aβ-induced synapse loss remains unknown. Therefore, we addressed this question using a primary culture of hippocampal neurons.

Method

The neuronal activity of cultured hippocampal neurons from mouse pups was increased using the chemogenetic technique designer receptors exclusively activated by designer drugs (DREADD). The cultured neurons were treated with Aβ, and synapse density was assessed by immunocytochemistry.

Results

Aβ decreased the synapse density probabl

SUBMITTER: Kono R 

PROVIDER: S-EPMC7292309 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature