Import of Aspartate and Malate by DcuABC Drives H₂/Fumarate Respiration to Promote Initial Salmonella Gut-Lumen Colonization in Mice.

ABSTRACT: Initial enteropathogen growth in the microbiota-colonized gut is poorly understood. Salmonella Typhimurium is metabolically adaptable and can harvest energy by anaerobic respiration using microbiota-derived hydrogen (H₂) as an electron donor and fumarate as an electron acceptor. As fumarate is scarce in the gut, the source of this electron acceptor is unclear. Here, transposon sequencing analysis along the colonization trajectory of S. Typhimurium implicates the C4-dicarboxylate antiporter DcuABC in early murine gut colonization. In competitive colonization assays, DcuABC and enzymes that convert the C4-dicarboxylates aspartate and malate into fumarate (AspA, FumABC), are required for fumarate/H₂-dependent initial growth. Thus, S. Typhimurium obtains fumarate by DcuAB

SUBMITTER: Nguyen BD 

PROVIDER: S-EPMC7292772 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature