GDF5 induces TBX3 in a concentration dependent manner - on a gold nanoparticle gradient.

ABSTRACT: Organs and tissues, such as cartilage and limbs, are formed during development through an orchestration of growth factors that function as morphogens. Examples of growth factors include growth differentiation factor 5 (GDF5) and transforming growth factors beta 1 and 3 (TGFβ-1 and TGFβ-3) which can specify creation of more than one cell type after forming a concentration gradient in vivo. Here, we studied the impact of morphogen gradients during differentiation of induced pluripotent stem cells (iPSCs) into the chondrocyte lineage. Cell budding zones, consisting of condensed cell aggregates, were observed only in gradients of GDF5. T-box transcription factor 3 (TBX3) was detected specifically in the budding zones (ranging from 500-1,500 particles/μm²) of nuclei and cell v

SUBMITTER: Andreasson L 

PROVIDER: S-EPMC7292926 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature