Dynamic regulation of chromatin organizer SATB1 via TCR-induced alternative promoter switch during T-cell development.

ABSTRACT: The chromatin organizer SATB1 is highly enriched in thymocytes and is essential for T-cell development. Although SATB1 regulates a large number of genes important for T-cell development, the mechanism(s) regulating expression of SATB1 during this process remain elusive. Using chromatin immune precipitation-seq-based occupancy profiles of H3K4me3 and H3Kme1 at Satb1 gene locus, we predicted four different alternative promoters of Satb1 in mouse thymocytes and characterized them. The expression of Satb1 transcript variants with distinct 5' UTRs occurs in a stage-specific manner during T-cell development and is dependent on TCR signaling. The observed discrepancy between the expression levels of SATB1 mRNA and protein in developing thymocytes can be explained by the differential translatabili

SUBMITTER: Patta I 

PROVIDER: S-EPMC7293019 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature